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KMID : 0356420000180020111
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Journal of Korean Andrology
2000 Volume.18 No. 2 p.111 ~ p.116
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Effect of Alpha-2 Receptor Blocker on Serotonin Release in Rat Hippocampal Slices
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Song Yun-Seob
Kim Min-Eui
Park Young-Ho
Ahn Young-Soo
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Abstract
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Purpose: It is known that alpha-2 receptor is found at the presynaptic serotonergic nervous system and a consequence of increased activation of alpha-2 receptor decreases the serotonin release. So, Alpha-2 blockers may have the effect on the release of serotonin. The major serotonergic innervation is found at the hippocampus. This study was performed to investigate the effect of the alpha-2 receptor blocker to serotonin release in rat hippocampus.
Materials and Methods: The hippocampus from the male rat brain was sliced. After 30 minutes preincubation in the normal buffer, the slices were incubated for 20 minutes in a buffer containing 0.1 ㎛ [3H]5-HT for uptake, and washed. After administration of alpha 2 receptor blockers, yohimbine (10-5 M), the release of [3H]5-HT into the buffer was measured, the radioactivities in each buffer and the tissue were counted and the results were expressed as a percentage of the total
activity. The value of released [3H]5-HT was expressed as percent of the value at 50 minutes when a steady state of [3H]5-HT release was obtained.
Results: After administration of yohimbine (10-5 M), the values(mean±SE,%) were 147.5±9.2 at 60 minutes and 143.8±7.3 at 70 minutes compared to the values of control group, 96.6±1.9 at 60 minutes and 89.6±2.3 at 70 minutes. The release was increased significantly after administration of yohimbine.
Conclusions: Alpha-2 receptor blockers increased the release of serotonin. It is suggested that the consideration of the increase of serotonin release by alpha-2 receptor blockers may be helpful to understand the effect of serotonin on sexual function.
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KEYWORD
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Alpha-2 receptor blockers, Serotonin, Hippocampus,
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